backBack to 4/2015
Technical Issues
4/2015 pp. 3-10

Pochodne kumaryny do roli molekularnych sensorów fluorescencyjnych do zastosowań w naukach life science

pdf Get full text pdf


Fluorescent spectroscopy is an important method in life science which has recently gained popularity. Fluorescent spectroscopy is widely used for example by the researches in the variety of biological structures, intercellular interactions, various kinds of biomolecules and also for understanding of the biochemical processes which take place inside the living organisms. For this purpose, an application of fluorescent sensors and searching for more efficient and more sensitive fluorophore for illustrating, labelling and detection, have become a developing trend in biochemistry, medicine, biology and also in many other chemistry researches. Nowadays, issues which are connected to fluorescence are mainly applied to the newest achievements in the field of fluorescent methods and measurement techniques and also to the development and applications of fluorescent probes, what is closely showed up in this article.

Key words

coumarin, fluorescence, molecular sensor, molecular probes, fluorophore


1. Suppan, P., Chemia i światło, Warszawa, 1981, PWN.

2. Baltrop, J.A., Coyle, J.D., Fotochemia, Warszawa, 1987, PWN.

3. Simons, J.P., Fotochemia i spektroskopia, Warszawa, 1976, PWN.

4. Nakabayashi, T., Wang, H., Kinjo, M., Application of fluorescence lifetime imaging of enhanced green fluorescent protein to intracellular pH measurements, Photochem. Photobiol. Sci., 2008, 7, pp. 668-670.

5. 6. Yinan, W., Yuanzong, L., Wenbao, C., Application of Fluorescence Resonance Energy Transfer Technique in Bioanalysis, Chinese Journal of Analytieal Chemistry, 1998, 04, 027.

6.  Pączkowski, J., Sondy fluorescencyjne jako narzędzie badawcze w chemii polimerów, Polimery, 2005, 50 (7-8), s. 520-529.

7. Scully A.D., Ostler, R.B., Phillips, D., Neill, P.O., Application of fluorescence lifetime imaging microscopy to the investigation of intracellular PDT mechanisms, Bioimaging, 1997, 5 (1), pp. 9-18.

8. (dostęp 2.02.2015).

9. Tsien R.Y., The green fluorescent protein, Annu. Rev. Biochem., 1998, 67, pp. 509-544.

10. Chalfie, M., Green fluorescent protein, Photochemistry and Photobiology, 1995, 62, 4, pp. 651-656.

11. Noga, E.J., Udomukusonsri, P., Fluorescein, A Rapid, Sensitive, Nonlethal Method for Detecting Skin Ulceration in Fish, Vet Pathol, 2002, 39, pp.726–731.

12. Mathew, T., Kundan, S., i in., Multiple Muscular Ventricular Septal Defects: Use of Fluorescein Dye to Identify Residual Defects, Ann Thorac Surg., 2014, 97, pp. 27–28. 

13. Wheelock, Ch.E., The Fluorescence of Some Coumarins1, Journal of the American Chemical Society, 1959, 81, p. 1348-1352.

14. Raju, B., Varadarajan, T.S., Spectroscopic studies of 7-diethylamino-3-styrylcoumarins,

J. Photochem. Photobio. A., 1995, 85, 3, pp. 263-267.

15. Chen, K.., Guo, Y., Lu, Z. Yang, B., Shi, Z., Novel coumarin-based fluorescent probe for selective detection of bisulfite anion in water, Chin. J. Chem., 2010, 28, 1, pp. 55-60.

16. Jung, H.S., Kwon, P.S., Lee, J.W., Kim, J.I., Hong, C.S., Kim, J.W., Yan, S., Lee, J.Y., Coumarin-derived Cu2+-selective fluorescence sensor:synthesis, mechanisms, and applications in living cells, J. Am. Chem. Soc., 2009, 131, 5, pp. 2008-2012.

17. Li J., Lin H., Cai Z., Lin, H. (2009). A novel coumarin-based switching-on fluorescent andcolorimetric sensor for F–. J. Lumin., 129, 5, pp. 501-505.