backBack to 4/2015
Technical Issues
4/2015 pp. 19-25

Molekularne sondy fluorescencyjne w biologii, biochemii i biotechnologii


pdf Get full text pdf

Abstract

Nowadays molecular fluorescent probes are widely used in many fields of science, primarily in biology, medicine and biotechnology. Particular emphasis on the development of technologies to cancer cells diagnosis and detection of genetic mutations led to numerous modification of luminescent fluorophores, which has resulted in a wide range of such compounds. Scientists from around the world are continuously trying to experience the mechanism of cells function and metabolic pathways. Certainly, it is affected by the fact that any analytical techniques using fluorescence operate easily and nondestructively. A phenomenon of fluorescence is observable by chromophore which absorbs energy at a certain wavelength and is able to emit electromagnetic radiation at another wavelength.

Key words

fluorescent probe, molecular biology, detection of proteins

References

1. Gonçalves, M.S.T., Fluorescent Labelling of Biomolecules with Organic Probes, Chemical Reviews, 2009, 109, pp. 190-212.

2. The Molecular Probes® Handbook - A Guide to Fluorescent Probes and Labeling Technologies, 2014, http://www.atdbio.com (dostęp 28.12.2014).

3. Tsien, R.Y., The Green Fluorescent Protein, Annu. Rev. Biochem., 1998, 67, pp. 509-544.

4. Geddes, C.D., Lakowicz, J.R., Reviews in Fluorescence, Springer, 2006, http://www.rcsb.org (dostęp 03.12.2014).