backBack to 3/2016
Technical Issues
3/2016 pp. 67-73

Optymalizacja parametrów pracy aparatury pomiarowej w analizie pierwiastkowej tkanek miękkich techniką ablacji laserowej z detekcją w spektrometrze mas z plazmą sprzężoną indukcyjnie

pdf Get full text pdf


Operating parameters of measurement equipment for the laser ablation of detection in the mass spectrometer with inductively coupled plasma were optimized. Optimization was employed in order to choose the conditions for carrying analyzes to ensure high analytical signal intensities and low values of relative standard deviation. Optimization was performed on a model sample with physicochemical properties similar to those of real samples of soft tissue. The following parameters underwent optimization: the energy of the laser pulse frequency, beam diameter and scanning speed. The selection of the optimal operating parameters was determined based on the intensity of the signal analysis, RSD value and visual evaluation of the process of ablation. Measurements were made using a quadrupole ICP-MS spectrometer (Elan DRC II PerkinElmer) with a laser ablation system (LSX-500 Cetac).

Key words

laser ablation, inductively coupled plasma mass spectrometry, optimization of operating parameters of instruments, soft tissue


1. Dean, R.J., 2005. Practical inductively coupled plasma spectrometry, Wiley, 2005.

2. Hare, D., Austin, Ch., Doble, P., Quantification strategies for elemental imaging of biological samples using laser ablation-inductively coupled plasma-mass spektrometry, Analyst, 2012, 137, pp. 1527–1537.

3. Hill, J.S., Inductively coupled plasma spectrometry and its applications, Wiley-Blackwell, 2007.

4. Jurowski, K., Buszewski, B., Piekoszewski, P., The analytical calibration in (bio) imaging/mapping of the metallic elements in biological samples – Definitions, nomenclature and strategies: State of the art., Talanta, 2015, Jan., 131, pp. 273–285.

5. Thomas, R., Practical guide to ICP-MS: A toutorial for begginers, CRC Press, 2013.

6. Żyrnicki, W., Borkowska-Burnecka, J., Bulska, E., Szmyd E., Metody analitycznej spektrometrii atomowej - teoria i praktyka, Wyd. Malamut, Warszawa 2010.